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A4 9: 15 ISOLATED WHOLE MOUSE HIPPOCAMPUS AND NEOCORTEX AS LIVE IN VITRO PREPARATIONS. Melissa L. Davies and R. David Andrew, Dept. Anatomy & Cell Biology, Queen`s University, Kingston, ON K7L-3N6 Brain slices as thick as 600 microns remain healthy for several hours through diffusion of O2, glucose and salts from the artificial cerebral spinal fluid that superfuses the tissue. The whole, isolated neocortex and hippocampus from young mouse 7-13 d.o. ; are approx. this thickness and so should be viable during artificial CSF superfusion. In this study, we confirmed this hypothesis and characterize both whole isolated preparations using extracellular electrophysiological recording and imaging of light transmittance LT ; . In particular we examined the anoxic depolarization AD ; , the initial cortical event in stroke that propagates in 3 dimensions but is necessarily confined in brain slices to two. Within 5 min of O2 glucose deprivation OGD ; , AD arises focally and expands as a sphere within neocortical or hippocampal gray. Micropipette placement to maximize evoked field potential amplitude is followed by OGD, eliciting a negative voltage shift that confirms AD generation both in whole hippocampus and neocortex. We are currently using 2-photon laser confocal microscopy to show relatively undamaged neuronal processes at the surface of whole preps compared to slice surfaces. Supported by the HSFO and the CIHR.
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REFERENCES 1. Evidence-based medicine. A new approach to teaching the practice of medicine. JAMA 1992; 268: 2420-5. Slawson DC, Shaughnessy AF, Bennett JH. Becoming a medical information master: feeling good about not knowing everything. J Fam Pract 1994; 38: 505-13. Shaughnessy AF, Slawson DC, Bennett JH. Becoming an information master: a guidebook to the medical information jungle. J Fam Pract 1994; 39: 489-99. Siwek J, Gourlay ML, Slawson DC, Shaughnessy AF. How to write an evidence-based clinical review article. Fam Physician 2002; 65: 251-8. Systems to rate the strength of scientific evidence. Summary, evidence report technology assessment: number 47. AHRQ publication no. 02-E015, March 2002. Agency for Healthcare Research and Quality, Rockville, Md. Accessed November 13, 2003, at: : ahrq.gov clinic epcsums strengthsum. htm. 6. Harris RP, Helfand M, Woolf SH, Lohr KN, Mulrow CD, Teutsch SM, et al. Current methods of the U.S. Preventive Services Task Force: a review of the process. J Prev Med 2001; 20 3 suppl ; : 21-35. 7. Clarke M, Oxman AD. Cochrane reviewers' handbook 4.2.0. The Cochrane Collaboration, 2003. Accessed November 13, 2003, at: : cochrane resources handbook handbook . 8. Gyorkos TW, Tannenbaum TN, Abrahamowicz M, Oxman AD, Scott EA, Millson ME, et al. An approach to the development of practice guidelines for community health interventions. Can J Public Health 1994; 85 suppl 1 ; : S8-13. 9. Briss PA, Zaza S, Pappaioanou M, Fielding J, Wright-De Aguero L, Truman BI, et al. Developing an evidence-based guide to community preventive services--methods. J Prev Med 2000; 18 1 suppl ; : 35-43. 10. Greer N, Mosser G, Logan G, Halaas GW. A practical approach to evidence grading. Jt Comm J Qual Improv 2000; 26: 700-12. Guyatt GH, Haynes RB, Jaeschke RZ, Cook DJ, Green L, Naylor CD, et al. Users' guides to the medical literature: XXV. Evidence-based medicine: principles for applying the users' guides to patient care. JAMA 2000; 284: 1290-6. Major cardiovascular events in hypertensive patients 13.
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ALASKA STATE LEGISLATURE SENATE HEALTH, EDUCATION AND SOCIAL SERVICES STANDING COMMITTEE March 21, 2005 2: p.m. MEMBERS PRESENT Senator Senator Senator Senator Senator Fred Dyson, Chair Gary Wilken, Vice Chair Lyda Green Kim Elton Donny Olson.
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A style of melodic trance is played with innumerable `little sounds' that exercise the participant's creativity. This state is ideal for the futuristic character that the rave assumes during the night with its fluorescent decoration and less accelerated music. To match this bodily and mental state of the ravers, the organizers look to offer water in the centre of the dance floor and provide shaded areas. The organization of the DJs is structured so that the quicker styles are played in the morning and the slower music in the afternoon and at night. The difference between Mack DJ ; and Dino DJ ; is that with one of them you fry, and in the other you melt, but both are excellent. F., festival-goer ; When you're melting, your head is spinning and all your thoughts are rushing at the same time; and when you connect with the music, another trip starts and your thoughts are lulled by these small sounds. It's always like that when you take acid, that trip and the feeling that your body is melting and you've become really heavy. It really is good. D., DJ ; . In parallel to these two categories, there are also the secondary drugs that accompany the participants the whole time. Cannabis, alcohol, tobacco, hashish and skunk are all auxiliary drugs shared by everyone. These drugs are recognized as inoffensive or not producing symptoms that need special care on the part of participants as occurs in the previous cases. These substances are suitable for any period of the festival and help stimulate contact between the participants. Drug consumption in general is also one of the main factors behind the interaction among festival goers. During the purchase, consumption and swapping of this kind of substance, I could perceive how relations are established. The drugs deemed to be light which I listed as secondary are typically consumed in groups and enable moments of conversation and relaxation during which 'native knowledge' on the consumption of psychoactive substances is transmitted. This knowledge also includes the recommendation of a moment of relaxation and rest for those who `fried' or `melted' during the party and claritin.
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